Humblest greetings to you all i hope everyone is as well as they can be. My intentions here are not to promote anything especially herbal remedies. My advice is that we are in the age of information therefore anything with due diligence can be researched. One oviously has to be aware of misinformation and propaganda. MD Anderson have done testing with oleander extract. As to FDA and Big Pharma that is just too big of a topic to talk about. Off label arketing done by Big Pharma is illegal but is still practiced, fundamentally for an industry there to aid humanity (is it, isn't it, i don't know) puts up a big red flag for me. Just recently ive read about anti depressants being linked with a lot of unimaginable behaviours and crimes. The british Medical Joural will only allow papers to be published if all research data is provided, this is a relativley new practice, as before companies would only publish favourable outcomes to their studies regardless of whether the negative outnumbered the positive. So what im trying to say is, one must use their best judgement or rely on the best judgement of others.
My advice is to do extensive research in whatever might interest you in aiding your physical and mental health. In my fathers case he was given 6 months to live and we were blessed by having him for 7 years post diagnosis and that was without surgical or pharmaceutical intervention. I'm 35 years old now and a father of two and in my short time i think i may have learned a thing or two. Things in this world are not always what they seem. therefore when one suggests "the rigorous testing's required for FDA approval" i'm sure there are many arguments for and against what the FDA does and how Big Pharma do there "testing". No one person or peaople have the answer to everything in this world, so to me when one is steadfast in their opinion over something that may or not be true seems irrational. The philosophy that helped my father and my family during our difficult time is that god has given us everything we might need. I praise the doctors and care staff around my father as they all played thier parts to the fullest, in part i believe because we were informed consumers, this website was crucial. I pray for all of you, that God gives us all strength to continue.
Thankyou all for your condolences, its comforting to know that others understand what losing a loved one feels like and the words to use to help.
After a courageous 7 year battle with this cancer our handsome loving father, husband and grandfather Mr Punna Singh Hayre passed away peacefully in hospice on September 19th 2012. In the last few months after having had an external stent placed and paracentsesis procedure done, my father had been in and out of the hospital numerous times. He would be in hospital for a week or two then come home, develop an infection and would be right back in the hospital. He did not have any time to recover between infections. He was fed up of going to the hospital repeatedly and wanted to be at home, at one point he leftthe hospital and started walking home. He said his appetite had completely diminished and he had no interest in eating food. He started using morphine for pain control in the last few weeks, something he had never used before, this medication had some very undesirable side effects. In the end he stopped eating and stopped drinking and slowly faded away over the course of about 13 days. We find comfort in that we stood beside him every step of the way and helped him in anyway possible. In the end he said he had made his peace with God and was ready to go and he did go. We did his cremation on Sept 23rd 2012. We are very proud of him, he never gave up until it became too much, he fought till the end. I miss him very much he was my best friend, i hope to see him again one day. Love you Dad always and forever.
My dad hasgone an incredible 7 years this August post diagnosis of cholangiocarcinoma (klatskin tumour). He is doing relatively well. He is a bit jaundiced right now and has some ascites in his abdomen. His liver function is not bad. His albumin levels are a bit low. He had internal stents for 6 1/2 years but now has an external drain. He has lost quite a bit of weight. He is at home with us where his wife, me (his son), my wife and my 2 little children look after him. Not sure what the future holds but we remain positive.
Thoughts and prayers with you all as always.
My father chose not to do chemo or radiation because in his opinion chemo and radiation kill healthy cells along with cancer cells. From the reading ive done most chemo drugs are carcinogens themselves and people that handle them do so with extreme caution. So someone who has cancer is treated with medicine that can cause cancer and that weakens ones immune system that appears to be contradictory. That is just my opinion though. With cholangiocarcinoma symptoms appear when the cancer is already in a late stage which is a disadvantage. my father has lived relatively well for almost seven years.
Well you can click on my name to see my posts regarding my father. My father had internal stents at first, for 6 and half years. They usually stayed open for long periods of time sometimes a year. Just this last month he had to have an internal/external drain put in. From diagnosis he retired from work, we juiced a lot of vegetables and fruit and basically nourished his body with everything it needed. However we became complacent because my father was feeling relatively well and was quite active and he stopped drinking the juices and stopped the vitamins. He did maintain a relatively healthy diet but his appetite was lower than what it had been prior to cancer. I think he was very cautious about whathe ate because he was afraid of his stents getting plugged. Stopping the juicing was a big mistake. my father only went to the hospital to get stent changes and only saw his oncologist once a year. They were a little shocked that he was still living every time they saw him. My father is 67 years old now. I have done a lot of reading, the information is out there you have to decide what is relevant and what isnt, cross check every bit of information. my belief is that God (or whoever) gave us everything we need to survive on this earth you just need to know how to use it. I feel that building ones immune system is very important. So basically lots of fresh organic fruit juices. Vitamins and mineral supplements especially the ones to protect the liver. Natural bile thinners such as lemon water and beetoot juice. Distilled water to drink, low sugar diet and only healthy fats. My father now takes digestive enzymes to help digest his food. After courses of antibiotics i give him good quality probiotics to help restore natural bacteria in his intestines. Because his bile drains to the outside (some may be draining inside) he drinks electrolyte water to replenish lost salts. It all depends on how he feels, somedays are good and some not so good. I think he is a little drained from fighting but then i put his grand kids in front of him and his will is restored. He is not afraid to die, he just doesnt want to suffer. when we first found out about his cancer we were all devestated and in shock knowing that prognosis is poor especially if surgery does not work. But now because it has been almost seven years we are all prepared (as much as one can be)
I hope this helps
Hello, sorry to hear that your father is sick and i understand how you feel. Try to stay positive and focused. My father is nearly seven years past diagnosis of klatskin tumor. Surgery for this type can be quite complicated (according to the literature and my fathers surgeon). They did try surgery on my father but said it could be fatal because the portal vein was involved, clear margins might be difficult to achieve and cancer often recurrs. My father's oncologist said that most chemo and radiation treatments are ineffective. My father chose no to do chemo or radiation. My father has had some setbecks, but he is still with us. You need to inform yourself about every possible way you can help your father. This website is a wealth of information. May God be with you and your father. Im sending my prayers.
Phase I Study of Oleandrin (Nerium Oleander Extract) in Combination With Carboplatin and Docetaxel in Patients With Advanced Non-Small Cell Lung Cancer
Basic Trial Information
Trial Contact Information
Oleandrin (Nerium Oleander) in Combination With Carboplatin and Docetaxel in Treating Patients With Advanced Non-Small Cell Lung Cancer
Basic Trial Information
Phase Type Status Age Sponsor Protocol IDs
Phase I Biomarker/Laboratory analysis, Supportive care Approved-not yet active 18 and over NCI MDA-2011-0147
I.To determine the maximum-tolerated dose (MTD) of sublingual (SL) dosing of oleandrin (nerium oleander; Anvirzel) in patients with advanced non-small cell lung cancer (NSCLC) treated with chemotherapy.
II.To evaluate the pharmacokinetics of carboplatin and docetaxel when administered concurrently with SL Anvirzel.
I.To evaluate the anti-inflammatory and immunomodulatory effects of SL Anvirzel during carboplatin and docetaxel chemotherapy in patients with advanced NSCLC.
II.To evaluate symptoms and quality-of-life outcomes, the incidence of grade 3-4 toxicities, dose reductions, dose delays, and completion of scheduled carboplatin and docetaxel chemotherapy with SL Anvirzel in patients with advanced NSCLC.
•Patients must have histologically or cytologically confirmed diagnosed non-small cell lung cancer (NSCLC) and be scheduled to receive four courses of carboplatin and docetaxel chemotherapyScheduled to begin carboplatin and docetaxel chemotherapy in the next 30 days
•Newly diagnosed or previously treated patient with NSCLC; previously treated patients are allowed to have any previous chemotherapy for the treatment of NSCLC
•See Disease Characteristics
•No patients receiving any other investigational agents
•Patients receiving any medications or substances that are inhibitors or inducers of CYP 3A4 are ineligible
•No patients using or scheduled to use bevacizumab during study period
•No current use of cardiac glycoside
•Eastern Cooperative Oncology Group (ECOG) performance status ≤ 2 (Karnofsky ≥ 60%)
•Life expectancy of greater than 6 months
•Leukocytes ≥ 3,000/mcL
•Absolute neutrophil count ≥ 1,500/mcL
•Platelets ≥ 100,000/mcL
•Total bilirubin within normal institutional limits
•Aspartate aminotransferase (AST)(serum glutamic oxaloacetic transaminase [SGOT])/alanine aminotransferase (ALT)(serum glutamic pyruvate transaminase [SGPT]) ≤ 2.5 times institutional upper limit of normal
•Creatinine within normal institutional limits OR creatinine clearance ≥ 60 mL/min
•Negative serum or urine pregnancy test in women of child-bearing potential
•Women of child-bearing potential and men must agree to use adequate contraception (hormonal or barrier method of birth control; abstinence) prior to study entry and for the duration of study participation
•Ability to understand and the willingness to sign a written informed consent document
•No history of allergic reactions attributed to compounds of similar chemical or biologic composition to cardiac glycosides
•No uncontrolled intercurrent illness including, but not limited to, ongoing or active infection, symptomatic congestive heart failure, unstable angina pectoris, cardiac arrhythmia, or psychiatric illness/social situations that would limit compliance with study requirements
•Not pregnant or breastfeeding
•HIV-positive patients on combination antiretroviral therapy are ineligible
•No uncontrolled or significant cardiovascular disease, including:A myocardial infarction within 6 months
Uncontrolled angina within 6 months
Congestive heart failure within 6 months, defined as New York Heart Classification II (NYHC-II)
Diagnosed or suspected congenital long QT syndrome
Any history of clinically significant ventricular arrhythmias (such as ventricular tachycardia, ventricular fibrillation, Wolff-Parkinson-White (WPW) syndrome, or torsade de pointes); prolonged QTc interval on pre-entry electrocardiogram (EKG) (> 450 msec); if the automated reading is prolonged (i.e., > 450 msec), the EKG should be manually over-read
Any history of second or third degree heart block (may be eligible if currently have a pacemaker)
Heart rate < 50 beats/minute OR sustained heart rate > 110 beats/minute on pre-entry EKG
MTD of SL dosing of Anvirzel in combination with chemotherapy
Pharmacokinetics of carboplatin and docetaxel when administered concurrently with SL Anvirzel
Anti-inflammatory effects of SL Anvirzel during carboplatin and docetaxel chemotherapy
Immunomodulatory effects of SL Anvirzel during carboplatin and docetaxel chemotherapy
Symptoms and quality-of-life outcomes based on MDASI-LC and SF-12 scores
Grade 3-4 toxicities at each course according to NCI CTCAE version 4.0
Dose reduction and delays at each course
This is a dose-escalation study of oleandrin (nerium oleander extract).
Patients receive oleandrin sublingually (SL) 3 times daily (TID) on days -7 to 0 of course 1 and then throughout each course of carboplatin IV and docetaxel IV. Treatment repeats every 21 days for 4 courses in the absence of disease progression or unacceptable toxicity.
Patients complete the MD Anderson Symptom Inventory-Lung Cancer (MDASI-LC) and the quality-of-life (SF-12) questionnaires at baseline and periodically during treatment.
Plasma and peripheral blood mononuclear cell samples are collected at baseline and periodically during treatment for biomarker, pharmacokinetic, and pharmacodynamic studies.
After completion of study treatment, patients are followed up for 4 weeks.
Trial Contact Information
Trial Lead Organizations
M. D. Anderson Cancer Center at University of Texas
Richard Lee, MD, FACP, Principal investigator Ph: 713-745-2889; 800-392-1611
Official Title A Phase I Study of the Combination of Carboplatin, Docetaxel, and Increasing Doses of Sublingual Anvirzel (Nerium Oleander) in Advance Non-Small Cell Lung Cancer
Trial Start Date 2012-05-01 (estimated)
Trial Completion Date 2013-05-01 (estimated)
Registered in ClinicalTrials.gov NCT01562301
Date Submitted to PDQ 2012-03-08
Information Last Verified
Case Report: Cholangiocarcinoma
A white female patient born in Jan. 25, 1962 experienced abdominal pain and weight loss. Obstructive jaundice then occurred. ERCP (endoscopic retrograde cholangiopancreatography) and CAT scan were performed a revealed a significant stricture of the common right and left hepatic ducts. A stent was implanted and the patient underwent surgery and was found to have a small cicatrising mass at the hilum which involved the port vein posterially with adjacent metastatic lymph nodes. Biopsies confirmed the presence of an infiltrating carcinoma consistent with primary carcinoma of the bile duct. A segment III bypass was then performed. The patient's jaundice settled but the patient was discharged from the hospital when it was determined that chemotheraphy and/or radiotheraphy were not likely to be of any benefit to her.
By the time that the patient presented to a hospice (approximately 3 months after onset of the original symptoms), she had lost approximately 42 pounds and was in very poor medical condition. The patient started NOE treatment in an initial regimen comprising a daily I.M. injection dosage of 0.3 cc which was increased to 0.5 cc after one week and maintained.
The patient's general medical condition improved gradually. After approximately 3 months of treatment, the patient had gained approximately 28 pounds in weight and a follow-up CAT scan revealed no tumoral mass. An ERCP was performed approximately two months later to remove the stent. The ERCP also confirmed the absence of a tumoral mass.
The patient was then placed on a maintenance regimen comprising sublingual adminstration of NOE extract 3×0.3 cc every day.
This product is intended solely as a food supplement to enhance general health, and is not intended to diagnose, treat, cure, or prevent any disease. Nothing listed here should be considered as medical advice for dealing with a given problem. You should consult your health care professional for individual guidance on specific health problems.
Sutherlandia OPC extract is made up of a patented and proprietary mixture of two herbal extracts which, in combination, have powerful immuno-modulatory and cytotoxic effects.
Topics in this section:
Sutherlandia Frutescens Sutherlandia Research
Nerium Oleander Toxicity Studies
Conclusion Possible Adverse Effects
Sutherlandia Frutescens, or “cancer bush” as it is known by the indigenous people of Southern Africa, has been used for centuries to treat a variety of ailments. Its popularity has been publicly acknowledged by its appearance on a South African postage stamp.
It has powerful immune boosting properties and has been classified as an adaptogen. (An adaptogen is an herbal substance or “tonic” that helps the body to adapt to environmental and internal stress by changing body metabolism.)
Sutherlandia is a flowering shrub of the pea family that grows up to 1.2m high and is found in its natural state only in the drier areas of the Western and Northern Cape provinces of Southern Africa
The use of medicinal preparations from the leaves has been recorded for the following conditions:
Blood Cleansing Cancer
Chronic Fatigue Syndrome Depression
Diabetes (type 2) Edema
Fever Gastric Ailments
Heartburn Hot Flushes
ME Peptic Ulcer
Rheumatism Rheumatoid Arthritis
Viral Hepatitis Wasting from cancer, TB and AIDS
It has also been used as an appetite stimulant for people wasted by disease though it does not stimulate the appetite of healthy people.
Research has shown that the active ingredients in Sutherlandia consist of a mixture of L-canavanine (an amino acid with documented anti-cancer, antiviral, anti-fungal and anti-bacterial action), pinitol (used in clinical settings to treat the wasting syndrome associated with cancer, TB and AIDS), gamma-aminobutyric acid (GABA - an inhibitory neurotransmitter that produces a sense of well-being), L-arginine (an antiviral agent), saponins and gamma sitosterol. Although the exact mechanism by which improvement of ailments is brought about is still relatively unknown, studies have shown the Sutherlandia could help diabetics by inhibiting inflammation and by its improvement of hyperglycemia. It has also been shown that the herb can positively affect the secretory function of the pancreas.
Apart from the papers mentioned below, a number of promising projects are presently being undertaken by various research institutions to test the efficacy of Sutherlandia Frutescens. It is a medical herb with a long history and well-documented research.
Chinkwo KA. - Sutherlandia frutescens extracts can induce apoptosis in cultured carcinoma cells. J Ethnopharmacol. 2005 Apr 8;98(1-2):163-70.
Fernandes AC, Cromarty AD, Albrecht C, van Rensburg CE. - The antioxidant potential of Sutherlandia frutescens. J Ethnopharmacol. 2004 Nov;95(1):1-5.
Harnett SM, Oosthuizen V, van de Venter M.- Anti-HIV activities of organic and aqueous extracts of Sutherlandia frutescens and Lobostemontrigonus. J Ethnopharmacol. 2005 Jan 4;96(1-2):113-9.
Ojewole JA. - Analgesic, antiinflammatory and hypoglycemic effects of Sutherlandia frutescens R. BR. (variety Incana E. MEY.) [Fabaceae] shoot aqueous extract. Methods Find Exp Clin Pharmacol. 2004 Jul-Aug;26(6): 409-16
Tai J, Cheung S, Chan E, Hasman D. - In vitro culture studies of Sutherlandia frutescens on human tumor cell lines. J Ethnopharmacol. 2004 Jul;93(1):9-19.
Oleander has been used for medical purposes for more than 2000 years. In biblical times it was known as the “desert rose”.
In Turkey, it has been used as a folk remedy for centuries.
During the 1960s, a Turkish doctor, Huseyin Ozel, saw that people using the remedy were mostly free of cancer and other serious diseases. He knew about the poisonous nature of the plant but soon discovered how to prepare the extract used as folk medicine. As head of the surgical department of the Mugla State Hospital in Turkey, he started experimenting with the extract and subsequently successfully used it as a treatment for cancer for more than 35 years. At the request of his son, Dr Ozel patented an aqueous extract of Nerium Oleander as ANVIRZEL. (US Patent #5,135,745) The Nerium Oleander extract used in the Sutherlandia OPC proprietary herbal mix is similar to the ANVIRZEL extract and the following excerpt is taken from the description of ANVIRZEL on the Internet: (the oral form of ANVIRZEL and Oleander Extract are the same)
Extensive laboratory and clinical experience indicate both cytotoxic and immunological activities for the drug. In developing a protocol for clinical trials, M.D. Anderson Cancer Center states, "It is thought that the polysaccharides present in Oleander Extract are capable of activating the immune cells, which in turn can recognize the epitopes present on the cancer cell surfaces, thereby enhancing the efficacy of the immune response. It is believed that the cytotoxic action present in the extract may be essential for synergistic activities. It is believed that the cytotoxic action both arrests tumor growth and prevents cancer cell reproduction as well as having and/or producing a Tumor Necrosis Factorlike activity. Research has proved conclusively that Oleander extract is active on dual pathways at the cellular level to both inhibit the growth of tumor cells (through the antiangiogenesis activity of the oleandrin) and to promote apoptosis of the tumor cells.
Extensive in vitro research has been conducted by Dr. Robert Newman, Chief of Pharmacology, M.D. Anderson Cancer Center (MDACC), Houston, Texas. Dr. Newman has tested Oleander Extract against a broad spectrum of human malignant cell lines, and has demonstrated that Oleander Extract has a high order of efficacy.
In addition to the research being conducted by M.D. Anderson Cancer Center, concurrent research is being conducted by Dr. Wendell Winters, a noted immunologist with the University of Texas Health Science Center in San Antonio,Texas. Dr. Winter's work has confirmed that Oleander Extract has been "shown to stimulate the immune system by stimulation of the function and capability of specific subsets of mononuclear cells." In addition, Dr. Winters' research has shown that Oleander Extract specifically stimulates T and B lymphocytes, the cell-mediated and the humoral mediated immune systems.
In April 2000, a USFDA approved study entitled "Phase I Study of ANVIRZEL in Patients with Advanced Solid Tumors" was commenced under the direction of Ronald Buckowski, M.D. at Cleveland Clinic in Cleveland, Ohio.
Because of its strong cytotoxic effect in combination with an equally strong immunomodulatory effect, Oleander Extract is indicated as a therapy, both primary and adjuvant, for cell proliferative disease (cancer), certain viral disease, and autoimmune/inflammatory disease. Clinical application of Oleander Extract in the United States, Ireland, and Honduras has demonstrated efficacy against various neoplastic disease, hepatic disease such as Hepatitis C, late and early stage HIV/AIDS, as well as autoimmune/inflammatory disease such as rheumatoid arthritis and psoriasis. The results have been determined both by the clinical practitioner and independent laboratory analysis using PET, CT scan, MRI, and hematological screening.
Using Oleander Extract Therapy, international clinicians have been treating patients suffering from the above referenced disorders on a compassionate use basis since 1997. Many of these patients were previously diagnosed as terminal. These clinicians have experienced a very high level of success with disease stabilization, partial remission, and complete remission, almost always accompanied by a very marked improvement in the patients' quality of life.
Dr. Anibal Villatoro of Tegucigalpa, Honduras, Former Executive Director of the Honduran Institute of Social Security (administrator of the public health system) has since January of 1999 been conducting a compassionate use trial with Oleander Extract for HIV (SIDA) patients in Tegucigalpa. His early results indicate a strong level of response to Oleander Extract therapy with a feeling of homeostasis (feeling of well being) and an improved quality of life, as well as significant improvement in their immune systems. (Reports that slipped out in late 1999 showed that Oleander Extract reversed AIDS, no matter what the phase of the disease, arthritis, psoriasis, hepatitis C, and even diabetes in some cases. Initially, Oleander Extract was thought to work only on cancers found early, however, very positive results have been found in people given just weeks to live. To top this all off, Oleander Extract seems to be the first cancer remedy to show positive results for leiomyosarcoma, probably the deadliest of cancers. Oleander extract also crosses the blood-brain barrier (like Poly-MVA) and gives hope to people with brain tumors.)
The "Common Thread" running through almost all of the clinical records of the patient population using Oleander Extract on a compassionate use basis has been the marked improvement in the "quality of life" of those patients. This includes, but is not limited to, homeostasis, marked improvement in pain management with elimination of or marked reduction in use of analgesics, positive response to antibiotics, increased appetite with concomitant weight gain, and increase in energy with reduction of fatigue.
There have been no reported adverse effects of the herb. A safety study, funded by the South African Medical Council, was conducted over a period of 3 months using 16 vervet monkeys. Behaviour of the animals was monitored and blood tests as well as various physiological evaluations were normal. No toxicity was found even at large dosages.
A toxicity study was performed by Southern Research Institute, Birmingham, AL, on 28 beagle dogs, and the study states, "No clinical signs of toxicity were noted in any of the dogs in this study..." Another lethality assessment of ANVIRZELTM in a murine (laboratory mouse) population was conducted by Southwest Research Institute, San Antonio, TX., and the study states, "On the day of NOI (Nerium Oleander Extract Injectible) administration and over a subsequent 14 day post dose period, none of the treated animals showed any pathological signs or ill effect of the injections as assessed by daily morbidity and mortality observations."
No reports of toxicity have been received from clinicians supervising patients experiencing Oleander Extract Therapy.
The herbal components that make up the Sutherlandia OPC mixture are individually both effective traditional remedies. The proprietary mix combines their known effects and the following people may obtain beneficial results from the product:
People with an impaired or suppressed immune system from any cause
People with a high occurrence of infectious diseases, including colds and flu
People with allergies or skin conditions, including skin cancer
People with various types of cancer
People undergoing chemotherapy and/or radiation
People over the age of 40 when the immune system starts to slow down due to the natural aging process
People affected by extra free radical production from external sources such as UV radiation, electromagnetic fields, poor nutritional habits and toxic chemicals
People with chronic diseases such as diabetes, chronic inflammation and chronic fatigue
Professional and amateur athletes, or anyone who works out intensively
People under severe physical or emotional stress
People with arteriosclerosis. Sutherlandia OPC helps draw extra cholesterol from the blood, thus preventing further plaque formation on the arterial walls
People with autoimmune diseases. Sutherlandia OPC helps the system to maintain homeostasis
People with HIV/AIDS
Possible Adverse Effects
Possible side effects from this herbal mix when used as a dietary supplement may be slight nausea and vomiting, diarrhea, pruritus, pain at a tumour site, tachycardia and arrhythmias. Because of its blood-thinning properties, people on medical blood-thinning preparations should consult their doctors before using the OPC. People on heart-active drugs, such as digoxin (Lanoxin®) or anti-arrhythmics should also speak to their doctors before using the mixture. No other drug interactions have been reported. Pregnant women should preferably not use the mixture due to its anti-angiogenesis effect. Other short term detoxification symptoms like a slight rash, runny nose, pimples, slightly painful joints, etc, may be experienced. The Sutherlandia in the mixture may cause a slight dryness in the mouth.
5ml (1 teaspoon) or 1 capsule three times per day with meals
(total of 15ml or 3 capsules per day).
The capsules contain the identical active ingredients as the liquid.
Titre du document / Document title
Vitamin K2-induced cell growth inhibition via autophagy formation in cholangiocellular carcinoma cell lines
Auteur(s) / Author(s)
ENOMOTO Masanobu ; TSUCHIDA Akihiko ; MIYAZAWA Keisuke ; YOKOYAMA Tomohisa ; KAWAKITA Hideaki ; TOKITA Hiromi ; NAITO Munekazu ; ITOH Masahiro ; OHYASHIKI Kazuma ; AOKI Tatsuya ;
Résumé / Abstract
Vitamin K2 (MK4) has antitumor effects on various types of cancer cell lines in vitro, and its efficacy has also been reported in clinical applications for patients with leukemia, myelodysplastic syndrome, and hepatocellular carcinoma (HCC). However, details of the mechanism of the antitumor effects of MK4 remain unclear. In the present study, we examined the antitumor effects of MK4 on cholangiocellular carcinoma (CCC) cell lines and its mechanism of action using the HL-60 leukemia cell line that exerts MK4-induced cell growth inhibition via apoptosis induction and cell cycle arrest as a control. MK4 exerted dose-dependent antitumor effects on all three types of CCC cell lines. However, apoptosis occurred in a smaller percentage of cells and there was less cell cycle arrest compared with other cancer cell lines studied previously, which suggested slight MK4-induced cell growth inhibition via apoptosis induction and cell cycle arrest. On the contrary, histopathological fidings showed a large number of cells containing vacuoles in their cytoplasm, and electron microscopic findings showed a large number of cytoplasmic autophagosomes and autolysosomes. These findings suggested evidence of autophagy-related cell death. Fluorescence microscopy following acridine orange staining revealed an increase in the number of cytoplasmic acidic vesicular organelles characteristic of autophagy. Moreover, there were few cells forming autophagic vesicles in the control group, while the percentage of cells containing vacuoles in the MK4-treated group increased with the duration of culture. These results suggested that, unlike in leukemia, gastric cancer, HCC, and other cancer cells, the antitumor effects of MK4 on CCC cells are induced via autophagy formation.
Revue / Journal Title
International journal of molecular medicine ISSN 1107-3756
Source / Source
2007, vol. 20, no6, pp. 801-808 [8 page(s) (article)]
Langue / Language
Editeur / Publisher
D.A. Spandidos, Athens, GRECE (1998) (Revue)
Mots-clés d'auteur / Author Keywords
cholangiocellular carcinoma ; vitamin K2 ; chemo- prevention ; autophagy ;
Localisation / Location
INIST-CNRS, Cote INIST : 27038, 35400017427155.0040
Nº notice refdoc (ud4) : 19692060
Not so much now hes been okay with that
My dad has been fighting for over 6 years now. He's been in the hospital for the last month. He had a stent inserted to open his pyloric valve as it had narrowed due to tumor, that explains why he couldnt eat, had acid reflux,always felt full and vomiited often. He's had and internal/external drain fitted which he has never had before they were always internal. GI doctor said he could not pass his scope through the pyloric stent and even when he had a look with a smaller scope he could not see the end of the existing internal bile stent thinking it might have tumor growing over it. Radiologist managed to get a stent through his existing internal stent with one end going into the duodenum and other end draining bile externally. Dads jaundice however still persists his eyes are really yellow and hes lost a lot of weight he is skin and bones. He had to have his drain replaced today as it was leaking. Dr said jaundice may stay the same as it appears the disease is progressing. He says dads liver function is stable but if he gets weaker and does not eat he may have 3 months. Dad is eating though he is still fighting. Ive told him that he shouldnt force himself but only eat if he wants to and he feels comfortable. My mum told me he eats in front of mJe cuz he thinks it will hurt my feelings as if he is giving up. Today he said he wants to fight he wants to come home. Its his grandson (my son) 2nd birthday this friday. Ive been taking him resource 2.0 to drink every day so does my mum and i help with his breakfast im mixing vitamin powder and some extra protein powder into his oatmeal. I told him that he needs to put some nutrients back into his body for the length of time he couldnt eat which was almost 2 to 3 weeks. Hes drinking hot water with juice of 1 lemon for his liver and to relieve the jaundice(hopefully). Im going to start giving him pedialyte for electrolytes he might be losing from the bile thats draining externally. Arrangements for hospice care at home have been made just in case. Ive been doing a lot of reading these past few days on how i can help him. Im thinking one last push whilst he wants to and he is able. So we stay strong and pray. It is physically and emotionally stressful and difficult. On a lighter note i came across something that might be of great interest to us all im not sure if anyone has come across this or not but do a google search for
Vitamin k2 inhibition of cholangiocellular carcinoma by autophage formation
Vitamin k therapy for cancer
Good luck to everyone and also as always thoughts and prayers for you all
Over the years this web site has helped me tremendously, it is a huge resource for people who are dealing with this type of cancer, it becomes very useful when people share their experiences. Heartfelt thanks to you all and i pray for each and everyone of you when i read your stories.
Dad was diagnosed with extrahepatic bile duct cancer in 2005/6. Resection was attempted but deemed too complicated. We sought no further treatment just good diet and family support. Dad has had regular stent changes and the occassional ascites drainage done and a bout with sepsis. 3 weeks ago he was unable to tolerate a few sips of water without wanting to vomit. ERCP revealed tumor obstructing his pyloric valve therefore his stomach was not emptying food or fluid into his small intestine. The doctor drained 3 litres of fluid from his stomach and also placed a stent in his pyloric valve. This gqave dad immediate relief and he started eating and drinking again. However the Dr was unable to pass his scope through the pyloric stent so was unable to clean his internal bile stents, he did pass through a smaller scope but was unable to see the stent protruding from the bile duct into the duodenum. He said that tumor seems to have covered the ducts. So dads been in hospital for 2 1/2 weeks. He had ascites drainage over the weekend and was scheduled to go for percutaneous bile drainage today as his bilirubin is up and his eyes are yellow. Fortunately the radiologist is the head of the dept. and was able to pass a stent percutaneously through his existing stents so bile can drain internally into the duodenum. Dad will probably have to stay in hosp. for a day or two then we will bring him home. One of his CT scans showed portal vein thrombosis which may be contributing to his ascites. Dr will put him on heparin and hopefully the clot will be reabsorbed, Dr said this could take upto 4 months. We have been informed about palliative care, mobile lab will come to take his blood to monitor his warfarin level. So hopefully now because dad can eat again we can fill him with lots of good stuff and put some weight back on him. He still feels strong, gets tired often but goes out in the garden to take alook at his veggies and plays with his grand kids. The fight continues.
My dads Indian piano is a small piano that u can sit on your lap and it has a bellow attached to the back of it that you use to pump into the piano to produce sound when you touch the keys.Its a bit like a harmonium.
Topic: Father Diagnosed with CC in Aug 2006- Update. (7 replies, posted in Introductions!)
My father was diagnosed with extrahepatic CC about the size of a walnut in Aug 2006. He was 61 years old then. Surgery was attempted but abandoned, Gallbladder was removed along with some lymph nodes which showed negative for CC. My father had no further treatment apart from love, compassion, prayers and lots of fresh organic fruit juice, good healthy diet and lots of laughs with his two grandchildren Jaya and Ajay. About 2 months ago he went to have his stent changed after which he developed sepsis, he had severe bloating and poor appetite and he lost about 40 Ibs.
He stayed in hospital for three weeks, had about 10 litres of fluid drained from his abdomen but eventually recovered. Fortunately last week we were told by his specialist that he could find no further occurence of ascites in his abdomen at his appt a week ago. This was good news because when ascites starts it usually means bad news and frequent trips to have the fluid drained. He is slowly regaining his appetite and remains active playing with his children and playing his Indian piano. We are due to go for a CT scan with contrast next month to see what the cancer is doing, he hasnt had a scan since 2007 because his health has been stable except for stent changes. This has been one hell of and emotional rollercoaster ride. So he has reached the 5 Years since diagnosis mile stone this October and we hope for another 5 years fingers crossed.
my dad drinks juiced beetroot ive read that this thins the bile
My father will be a four year survivor this october.He was diagnosed with extrahepatic cholangiocarcinoma klatskin tumor.No Surgery no chemo and no radiation.
Re: Dad diagnosed with inoperable klatskin Tumour oct 2006 (16 replies, posted in Introductions!)
Sorry for not replying earlier. My father did now want to do chemotherapy because he understands how it can ravish your healthy cells as well as the tumor.What we did was bought a high quality juicer and juiced organic fruits and vegetables mainly carrots, beets and green veggies like cabbage or spinach.Supplements he took were beta carotene, multi vitamin tablets and a few other supplements like selenium.He did this for almost a year and it improved his health in general and made him stronger and healthier.He stopped taking the juices eventually because he didnt like them but he does continue to eat a lot of fresh fruit and vegetables.He understands his body quite well now and knows what he can and cannot tolerate.He does not eat fatty foods at all.
My e mail address is email@example.com
Re: Dad diagnosed with inoperable klatskin Tumour oct 2006 (16 replies, posted in Introductions!)
The e mail is correct
and thank you all for you replies i just wanted people to know that although it is hard when a loved one is sick that we all need to have faith and empower ourselves.
Topic: Dad diagnosed with inoperable klatskin Tumour oct 2006 (16 replies, posted in Introductions!)
Hi everyone, Ive been reading posts on this website for almost 4 years now since my 61 year old father now 65 was diagnosed with cc.His story started with itching and then the onset of jaundice.After a few weeks he finally went for ultrasound and bloodwork and ERCP showed blocked ducts.About 2-3 months later he saw a liver specialist at Vancouver General Hospital who decided he would do exploratory surgery.We still had to wait another few months.He required a few stent changes in the meantime.Since his problems started i started looking into the ways that people deal with their cancer and started doing some of those things with him.We bought a good juicer and made him lots of fresh fruit juices which he tolerated reasonably well.Next was minerals,vitamins and other good supplements.We told him to stop working which he did and to have a positive attitude no matter what the outcome.It was an emotional rollercoaster for me,my mum and my wife.The day of the surgery arrived, the surgeon came to us after a few hours and determined he did now want to proceed with the surgery do to its complexity. no guaranteed cure and that my fathers quality of life may suffer.He told us that the tumour was extrahepatic and about the size of a walnut and near the portal vein.No mets was seen, the gallbladder was removed and lymph nodes were negative.Well he came home and we carried on with the same regimen of juice and supplements and its been nearly 4 years now.He has seen the birth of his first grandchild and will soon see his second.He does what he wants to enjoy himself and remains positive.Subsequent CT scans have not shown growth or spread of tumour and his stent has remained open for a long time now.I know that these tumours are all different in nature but this is our story.With prayers and positivity and love our family has gone through this tough time, we dont know how the story will end but for now everything is okay.He did not do any chemotherapy.My advice from my experience is to remain positive and educate yourself on what cancer is and how the body through love prayer and good foods can help fight ailments. Good luck to everyone my thoughts and prayers are always with you.Im an only child, im 32 and this site helped me in my time of desperation so thank you to Cholangiocarcinome.org.