Topic: Curcumin Study

Curcumin suppresses proliferation and induces apoptosis in human biliary cancer cells through modulation of multiple cell signaling pathways.
Prakobwong S, Gupta SC, Kim JH, Sung B, Pinlaor P, Hiraku Y, Wongkham S, Sripa B, Pinlaor S, Aggarwal BB.
Source

Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Abstract

Cholangiocarcinoma (CCA) is a tumor with poor prognosis that is resistant to all currently available treatments. Whether curcumin, a nutraceutical derived from turmeric (Curcuma longa), has potential therapeutic activity against human CCA was investigated using three CCA cell lines (KKU100, KKU-M156 and KKU-M213). Examination of mitochondrial dehydrogenase activity, phosphatidylserine externalization, esterase staining, caspase activation and poly-adenosine diphosphate ribose polymerase cleavage demonstrated that curcumin inhibited proliferation of and induced apoptosis in these biliary cancer cells. Colony-formation assay confirmed the growth-inhibitory effect of curcumin on CCA cells. When examined for the mechanism, curcumin was found to activate multiple cell signaling pathways in these cells. First, all CCA cells exhibited constitutively active nuclear factor (NF)-κB, and treatment with curcumin abolished this activation as indicated by DNA binding, nuclear translocation and p65 phosphorylation. Second, curcumin suppressed activation of signal transducer and activator of transcription-3 as indicated by decreased phosphorylation at both tyrosine(705) and serine(727) and inhibition of janus kinase-1 phosphorylation. Third, curcumin induced expression of peroxisome proliferator-activated receptor gamma. Fourth, curcumin upregulated death receptors, DR4 and DR5. Fifth, curcumin suppressed the Akt activation pathway. Sixth, curcumin inhibited expression of cell survival proteins such as B-cell lymphoma-2, B-cell leukemia protein xL, X-linked inhibitor of apoptosis protein, c-FLIP, cellular inhibitor of apoptosis protein (cIAP)-1, cIAP-2 and survivin and proteins linked to cell proliferation, such as cyclin D1 and c-Myc. Seventh, the growth inhibitory effect of curcumin was enhanced in the IκB kinase-deficient cells, the enzyme required for nuclear factor-kappaB activation. Overall, our results indicate that curcumin mediates its antiproliferative and apoptotic effects through activation of multiple cell signaling pathways, and thus, its activity against CCA should be further investigated.

PMID:
    21325634
    [PubMed - indexed for MEDLINE]
PMCID: PMC3165121
    [Available on 2012/9/1]

Please know that my personal opinion is not intended nor implied to be a substitute for professional medical advice. If  provided, information are for educational purposes.Consult doctor is a MUST for changing of treatment plans.

2 (edited by eli Fri, 09 Dec 2011 08:15:56)

Re: Curcumin Study

It's an exciting sounding study. But keep in mind, it was done in a dish. I only read the abstract and from the sounds of it, they applied curcumin directly to CCA cells.

If you plan to take curcumin orally, review what Wikipedia has to say about curcumin bioavailabily:

http://en.wikipedia.org/wiki/Curcumin#Bioavailability

FWIW, my wife currently takes 95% curcumin supplement with piperine ("bioperine"). I'm thinking about buying two other formulations mentioned on Wikipedia: Longvida and Meriva. It's very hard to tell which of these formulations works best. The "no-regrets" solution is to alternate between them.

Re: Curcumin Study

Hi,
What else of the supplements are your wife on?
Could you share with us?
God bless.

Please know that my personal opinion is not intended nor implied to be a substitute for professional medical advice. If  provided, information are for educational purposes.Consult doctor is a MUST for changing of treatment plans.

4 (edited by eli Sat, 10 Dec 2011 12:42:20)

Re: Curcumin Study

Hi Percy,

The list of supplements is not very long at the moment. Supplements are very frustrating to research. As you know, the evidence is all over the place. Lots of studies in a dish or in mice/rats. Human studies are often of very poor quality (small sample, non-randomized, open label, etc). For most supplements, I can find a bunch of studies that directly contradict each other. Studies that are specific to CCA are few and far in between.

Anyway, here's the list.

1. 95% curcumin with bioperine

http://www.swansonvitamins.com/SWH084/ItemDetail

2. Shiitake mushroom extract

http://www.swansonvitamins.com/SWH121/ItemDetail

3. Maitake mushroom extract

http://www.swansonvitamins.com/MSH005/ItemDetail

Once our current supply runs out, I think I will replace it with Maitake Gold 404

http://www.maitakegold.com/

Sloan-Kettering selected this formula for a clinical trial. The company that markets Maitake Gold has Dr. Nanba on board. He is the Japanese doctor who did the original research on Maitake.

4. Calcium supplement with Vitamin D3. Not cancer related.

5. Vitamin D3, to top up the daily dose. We get very little sunshine this time of year. I've seen a few studies that linked higher cancer rates in northern countries to lack of Vitamin D. The AntiCancer book is also big on D3.

6. Vitamin B12. Again, not cancer related. We eat very little meat. B12 deficiency is common among vegetarians. The bottle says it also helps with fat digestion (I could not verify this claim).

We might add Astragalus Extract and Milk Thristle in the near future. I'm still researching them.

Best wishes,
Eli

Re: Curcumin Study

Good day,

I am bringing up and older post to ask this community if there have been any further developments or personal experiences using Curcumin supplements ?

My sister-in-law has a colleague who is a scientist in the medical research field. She suggested my wife could benefit from taking a curcumin supplement. She suggested Meriva, a bioavailable version also know as 'curcumin phytosome.'

We are going to pose the question to our Oncologist since Lynn is currently on the Gem/Cis regimen. In another post from last year, Percy provided a link to an article which suggested they could work together.
http://www.cholangiocarcinoma.org/punbb … hp?id=9707

Thanks, in advance, for your comments.
Carl

Re: Curcumin Study

Hi,
I try to look it up on pub med but not much to be found.
One link interested me but of no immediately value to all of us,Carl, I think you can continue looking thru on the right hand column for additional articles
I think if you really like to use the herb as most people from India do( some of my co- worker do use curcumin every day as spices in their diet). I do not think it will hurt anyway.
But as Eli said in the message before, it is like the Chinese Herbal medicine, difficult to really get the active ingredient  to study like western medication do,
It is a research required lot of time and produced very little in the current method of testing and inventing new drugs.

http://www.ncbi.nlm.nih.gov/pubmed/23845850

God bless.

Please know that my personal opinion is not intended nor implied to be a substitute for professional medical advice. If  provided, information are for educational purposes.Consult doctor is a MUST for changing of treatment plans.

Re: Curcumin Study

Carl...in addition to Percy's comments made I would like to add the not so pleasant site of herbal research.  Unless the herbal structures can be included in the drug development and then patented, Pharma. Industry is not interested in producing products with little or no financial return. 
Hugs,
Marion

THIS INFORMATION IS NOT INTENDED NOR IMPLIED TO BE A SUBSTITUTE FOR PROFESSIONAL MEDICAL ADVICE. YOU SHOULD ALWAYS SEEK THE ADVICE OF YOUR PHYSICIAN OR OTHER QUALIFIED HEALTH CARE PROVIDER

Re: Curcumin Study

Two sources which promote the cancer fighting value of curcumin.

http://www.biomedcentral.com/1472-6882/12/23

The next is a podcast from Johns Hopkins, not specific to ICC.

http://media.hopkinsmedicine.org/#/podc … %20Cancer/


Carl

9 (edited by PCL1029 Sat, 17 Aug 2013 16:19:27)

Re: Curcumin Study

Hi, Carl ,
for entertaining purpose, I used my old balance for making special formulation medications in the good old days and based on figure 8 of the study,the lowest dose of CUM using 1000mg/kg of body weight and thru subcutaneous route
(the first link); I hand measured using the balance to measure out how many grams actually it is equal to the curry powder that i used for cooking.The answer  of course is depending on the dryness and the actual content of the active ingredient turmeric in the curry power.
The answer for my kind of curry power by oral route is equal to 10.75 tablespoonful(15ml=1 tablespoonful) or 75gm will be equal to the suggestion for the low end of curcumin dose of 1000mg/kg. That is a lot of curry power for cooking one or two dish for dinner.
I also find some interesting reading(see below); and if it is true, then we can add black pepper power to enhance the bio availability of turmeric.

"Curcumin is the yellow pigment associated with the curry spice, Turmeric, and to a lesser extent Ginger. It is a small molecule that is the prototypical 'curcuminoid', and has effects similar to other polyphenols but unique in a way as it is a different class of polyphenol (relative to the other classes of 'flavonoid', 'stilbene', etc.)

It exerts potent anti-inflammatory effects, and these anti-inflammatory effects seem to be quite protective against some form of cancer progression. However, curcumin has additional anti-cancer effects that are independent of its anti-inflammatory effects and thus is a heavily researched molecule for both cancer prevention and treatment.

Other areas of interest as it pertains to curcumin are alleviating cognitive decline associated with aging, being heart healthy by both electrical means and reducing lipid and plaque levels in arteries, and both reducing the risk of diabetes and being a good treatment for the side-effects associated with diabetes.

It has a poor oral bioavailability (a low percentage of what you consume is absorbed) and thus should be enhanced with other agents such as black pepper extract, called piperine. This is unless you want the curcumin in your colon (as it is a colon anti-inflammatory and can help with digestion), in which case you wouldn't pair it with an enhancement.

Doses up to 8g curcuminoids in humans have been shown to not be associated with much adverse effects at all, and in vitro tests suggest curcumin has quite a large safety threshold.

Another study link if interested:

http://meetinglibrary.asco.org/content/105958-133

God bless.

Please know that my personal opinion is not intended nor implied to be a substitute for professional medical advice. If  provided, information are for educational purposes.Consult doctor is a MUST for changing of treatment plans.

Re: Curcumin Study

Percy,

As always, thanks for taking time to research and respond. Something tells me there is value with curcumin. Lynn is adding turmeric to her daily diet, i.e. no supplements, but adding curcumin to her daily diet. Even a little each day seems like it will add value.

I hope you are doing well my friend. You have such great insight.

Take care,
Carl

Re: Curcumin Study

Thanks for that Percy and Carl. There is quite a lot of stuff out there on curcumin if you want to read further on it. I hope that Lynn gets on well with this Carl and please let us know how things go with this.

Best wishes to you both,

Gavin

Any advice or comments I give are based on personal experiences and knowledge and are my opinions only, they are not to be substituted for professional medical advice. Please seek professional advice from a qualified doctor or medical professional.