Topic: About Gene Amplification

Hi, everyone,

if you are interest ,below is part of the articles about Gene amplification.

1. Citation: Mukherjee K, Storici F (2012) A Mechanism of Gene Amplification Driven by Small DNA Fragments. PLoS Genet 8(12): e1003119. doi:10.1371/journal.pgen.1003119

DNA amplification is defined as a molecular process resulting in copy number increase of a discrete chromosomal DNA region. DNA amplification is found in many tumors, it is associated with several neuropathies and it can affect the susceptibility to certain diseases, such as systemic lupus erythematosus [1], [2]. It is believed that DNA copy number increase is a major molecular mechanism driving oncogenesis in many kinds of cancer, and it affects tumor progression and clinical outcome [3]. Gene amplification can in fact minimize the efficacy of drugs via overproduction of a protein that may be a drug target or via overproduction of a factor, which inactivates or eliminates the drug [4], [5]. DNA amplification, together with DNA copy number reduction is a major source of genetic variation, which is not necessarily always pathogenic, but which can lead to polymorphisms between individual genomes in humans and other organisms [6]–[14].....


2.Citation: Slack A, Thornton PC, Magner DB, Rosenberg SM, Hastings PJ (2006) On the Mechanism of Gene Amplification Induced under Stress in Escherichia coli. PLoS Genet 2(4): e48. doi:10.1371/journal.pgen.0020048

Gene amplification is the reiteration of a segment of a genome. It is a manifestation of genomic instability that is found in many tumors, notably some cases of neuroblastoma and some breast cancer in which it is associated with poor prognosis [1,2], and that arises during tumor progression in many others [1,2]. Amplification (and reduction) of genomic segments is now also appreciated to be among the most common of sequence variations, both pathogenic and polymorphic, between individual human genomes [3–5]. Amplification also occurs in microbes, in which it is implicated in the evolution of pathogenesis and antibiotic resistance [6]....
....DNA amplification can occur in response to stress. In Escherichia coli bacteria, starvation stress provokes amplifications that can allow E. coli ultimately to adjust to the starvation condition. This study elucidates several aspects of the mechanism underlying these stress-provoked amplifications. The data suggest a new model in which DNA replication stalls during starvation, and the end of the new DNA jumps to another stalled replication fork to create a duplicated DNA segment. The duplication can then amplify to many copies by genetic recombination. This model, if correct, can explain how stress provokes these genome rearrangements—by replication stalling. The general model may be useful for other long-distance genome rearrangements in many organisms. Stress can cause rapid and profound changes in the genome, some of which can give cells an advantage—this paper helps to explain how.----

God bless.

Please know that my personal opinion is not intended nor implied to be a substitute for professional medical advice. If  provided, information are for educational purposes.Consult doctor is a MUST for changing of treatment plans.

Re: About Gene Amplification

Thanks for these Percy.

Any advice or comments I give are based on personal experiences and knowledge and are my opinions only, they are not to be substituted for professional medical advice. Please seek professional advice from a qualified doctor or medical professional.

Re: About Gene Amplification

Thanks much, Percy. 
Hugs,
Marion

THIS INFORMATION IS NOT INTENDED NOR IMPLIED TO BE A SUBSTITUTE FOR PROFESSIONAL MEDICAL ADVICE. YOU SHOULD ALWAYS SEEK THE ADVICE OF YOUR PHYSICIAN OR OTHER QUALIFIED HEALTH CARE PROVIDER

Re: About Gene Amplification

Hi, Marion,

The gene amplification and overexpression are important in case of  patients have done the Chemotherapy sensitivity report or the"next-generation gene sequence" report. You will understand why the oncologist  choose the medication he intend to for you, but if you know the difference between amplification and overexpression even in simple terms, you can question the oncologist why not use  the other medication and let  him or her explaining to you.. A good oncologist will compare the results in the reports and choose the one that best for you, others just pick what the report said. Nothing wrong with that, but as you know, the oncologist knows if you know what you are talking about,they will look into your case more closely and carefully  and who will be benefit for that? you the patient.
And that is my intention to post the above.

Please know that my personal opinion is not intended nor implied to be a substitute for professional medical advice. If  provided, information are for educational purposes.Consult doctor is a MUST for changing of treatment plans.

Re: About Gene Amplification

Thank you so much for these and yesterday's post on the same topic.  This gives me some ammunition as I go for my Oncology appointment today.

PCL:  the oncologist knows if you know what you are talking about, they will look into your case more closely and carefully and who will be benefit for that? You the patient.

There have been times when I think he gives me answers that he believes will placate me.  And sometimes I know he is giving me a line of hooey, but I don't have the information to back up my argument.  I have learned to go into an appointment with papers in hand to support a point I want to make.

My oncologist already thinks I am a bit of a PIA, but in a good way he says.  This will only strengthen that argument...thanks to you!

Renée